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排序方式: 共有1119条查询结果,搜索用时 31 毫秒
61.
Achim von Bomhard Alexander Els?sser Lucas Maximilian Ritschl Silke Schwarz Nicole Rotter 《PloS one》2016,11(2)
Vitrification of endothelial cells (MHECT-5) has not previously been compared with controlled slow freezing methods under standardized conditions. To identify the best cryopreservation technique, we evaluated vitrification and standardized controlled-rate -1°C/minute cell freezing in a -80°C freezer and tested four cryoprotective agents (CPA), namely dimethyl sulfoxide (DMSO), ethylene glycol (EG), propylene glycol (PG), and glycerol (GLY), and two media, namely Dulbecco''s modified Eagle medium Ham’s F-12 (DMEM)and K+-modified TiProtec (K+TiP), which is a high-potassium-containing medium. Numbers of viable cells in proliferation were evaluated by the CellTiter 96® AQueous One Solution Cell Proliferation Assay (Promega Corporation, Mannheim, Germany). To detect the exact frozen cell number per cryo vial, DNA content was measured by using Hoechst 33258 dye prior to analysis. Thus, results could be evaluated unconstrained by absolute cell number. Thawed cells were cultured in 25 cm2 cell culture flasks to confluence and examined daily by phase contrast imaging. With regard to cell recovery immediately after thawing, DMSO was the most suitable CPA combined with K+TiP in vitrification (99 ±0.5%) and with DMEM in slow freezing (92 ±1.6%). The most viable cells in proliferation after three days of culture were obtained in cells vitrificated by using GLY with K+TiP (308 ±34%) and PG with DMEM in slow freezing (280 ±27%). 相似文献
62.
Gutierrez E Tkachenko E Besser A Sundd P Ley K Danuser G Ginsberg MH Groisman A 《PloS one》2011,6(9):e23807
Substrate rigidity profoundly impacts cellular behaviors such as migration, gene expression, and cell fate. Total Internal Reflection Fluorescence (TIRF) microscopy enables selective visualization of the dynamics of substrate adhesions, vesicle trafficking, and biochemical signaling at the cell-substrate interface. Here we apply high-refractive-index silicone gels to perform TIRF microscopy on substrates with a wide range of physiological elastic moduli and simultaneously measure traction forces exerted by cells on the substrate. 相似文献
63.
Rose AJ Díaz MB Reimann A Klement J Walcher T Krones-Herzig A Strobel O Werner J Peters A Kleyman A Tuckermann JP Vegiopoulos A Herzig S 《Cell metabolism》2011,14(1):123-130
Systemic bile acid (BA) homeostasis is a critical determinant of dietary fat digestion, enterohepatic function, and postprandial thermogenesis. However, major checkpoints for the dynamics and the molecular regulation of BA homeostasis remain unknown. Here we show that hypothalamic-pituitary-adrenal (HPA) axis impairment in humans and liver-specific deficiency of the glucocorticoid receptor (GR) in mice disrupts the normal changes in systemic BA distribution during the fasted-to-fed transition. Fasted mice with hepatocyte-specific GR knockdown had smaller gallbladder BA content and were more susceptible to developing cholesterol gallstones when fed a cholesterol-rich diet. Hepatic GR deficiency impaired liver BA uptake/transport via lower expression of the major hepatocyte basolateral BA transporter, Na(+)-taurocholate transport protein (Ntcp/Slc10a1), which affected dietary fat absorption and brown adipose tissue activation. Our results demonstrate a role of the HPA axis in the endocrine regulation of BA homeostasis through the liver GR control of enterohepatic BA recycling. 相似文献
64.
Hauser S Bickel L Weinspach D Gerg M Schäfer MK Pfeifer M Hazin J Schelter F Weidle UH Ramser J Volkmann J Meindl A Schmitt M Schrötzlmair F Altevogt P Krüger A 《PloS one》2011,6(4):e18989
Tumour-specific splicing is known to contribute to cancer progression. In the case of the L1 cell adhesion molecule (L1CAM), which is expressed in many human tumours and often linked to bad prognosis, alternative splicing results in a full-length form (FL-L1CAM) and a splice variant lacking exons 2 and 27 (SV-L1CAM). It has not been elucidated so far whether SV-L1CAM, classically considered as tumour-associated, or whether FL-L1CAM is the metastasis-promoting isoform. Here, we show that both variants were expressed in human ovarian carcinoma and that exposure of tumour cells to pro-metastatic factors led to an exclusive increase of FL-L1CAM expression. Selective overexpression of one isoform in different tumour cells revealed that only FL-L1CAM promoted experimental lung and/or liver metastasis in mice. In addition, metastasis formation upon up-regulation of FL-L1CAM correlated with increased invasive potential and elevated Matrix metalloproteinase (MMP)-2 and -9 expression and activity in vitro as well as enhanced gelatinolytic activity in vivo. In conclusion, we identified FL-L1CAM as the metastasis-promoting isoform, thereby exemplifying that high expression of a so-called tumour-associated variant, here SV-L1CAM, is not per se equivalent to a decisive role of this isoform in tumour progression. 相似文献
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The reactions of the Keplerate super cluster [Mo132O372(CH3CO2)30(H2O)72]42− with a Cu(II) source and an organonitrogen donor in methanol/DMF solutions yielded a series of bimetallic organic-inorganic oxide hybrid materials, including the molecular species [Cu(phen)2MoO4] (1) and [{Cu(terpy)}2(MoO4)2] (2) and a series of materials constructed from the tetranuclear building block {Mo4O10(OMe)6}2−: the molecular [{Cu2(phen)2(O2CCH3)2 (MeOH)}Mo4O10(OMe)6] (3), [{Cu(terpy)(O2CCH3)}2Mo4O10(OMe)6] (4) and [{Cu(terpy)Cl}2Mo4O10(OMe)6] (5), the one-dimensional phases [{Cu(bpy)(HOMe)2}Mo4O10(OMe)6] (6), [{Cu(bpy)(DMF)2}Mo4O10(OMe)6] (7), [{Cu(bpa)(DMF)2}Mo4O10(OMe)6] (8), [{Cu(phen)(DMF)2}Mo4O10(OMe)6] (9) and [{CuCl(dpa)}2Mo4O10(OMe)6] (10), and the two-dimensional material [{Cu2(DMF)2(pdpa)}{Mo4O10(OMe)6}2] (11). When methanol is replaced by the tridentate alkoxide tris-methoxypropane (trisp), the {Mo2O4(trisp)2}2− cluster building block is observed for [Cu(phen)Mo2O4(trisp)2] (12), [Cu(bpa)(DMF)Mo2O4(trisp)2] (13) and [{Cu(bpy)(NO3)}2Mo2O4(trisp)2] (14). 相似文献
67.
von Boehmer L Keller L Mortezavi A Provenzano M Sais G Hermanns T Sulser T Jungbluth AA Old LJ Kristiansen G van den Broek M Moch H Knuth A Wild PJ 《PloS one》2011,6(7):e21366
The cancer-testis (CT) family of antigens is expressed in a variety of malignant neoplasms. In most cases, no CT antigen is found in normal tissues, except in testis, making them ideal targets for cancer immunotherapy. A comprehensive analysis of CT antigen expression has not yet been reported in prostate cancer. MAGE-C2/CT-10 is a novel CT antigen. The objective of this study was to analyze extent and prognostic significance of MAGE-C2/CT10 protein expression in prostate cancer. 348 prostate carcinomas from consecutive radical prostatectomies, 29 castration-refractory prostate cancer, 46 metastases, and 45 benign hyperplasias were immunohistochemically analyzed for MAGE-C2/CT10 expression using tissue microarrays. Nuclear MAGE-C2/CT10 expression was identified in only 3.3% primary prostate carcinomas. MAGE-C2/CT10 protein expression was significantly more frequent in metastatic (16.3% positivity) and castration-resistant prostate cancer (17% positivity; p<0.001). Nuclear MAGE-C2/CT10 expression was identified as predictor of biochemical recurrence after radical prostatectomy (p = 0.015), which was independent of preoperative PSA, Gleason score, tumor stage, and surgical margin status in multivariate analysis (p<0.05). MAGE-C2/CT10 expression in prostate cancer correlates with the degree of malignancy and indicates a higher risk for biochemical recurrence after radical prostatectomy. Further, the results suggest MAGE-C2/CT10 as a potential target for adjuvant and palliative immunotherapy in patients with prostate cancer. 相似文献
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Schlapbach A Feifel R Hawtin S Heng R Koch G Moebitz H Revesz L Scheufler C Velcicky J Waelchli R Huppertz C 《Bioorganic & medicinal chemistry letters》2008,18(23):6142-6146
Pyrrolo-pyrimidones of the general structure 1 were synthesized and evaluated for their potential as MK2 inhibitors. Potent derivatives were discovered which inhibit MK2 in the nanomolar range and show potent inhibition of cytokine release from LPS-stimulated monocytes. These derivatives were shown to inhibit phosphorylation of hsp27, a downstream target of MK2 and are modestly selective in a panel of 28 kinases. 相似文献